Effect of rates of freezing, thawing and level of glycerol on the survival of bovine spermatozoa in straws.
نویسندگان
چکیده
Extended semen from six Hereford bulls was placed in .25-ml Continental straws and frozen in the vertical position. Treatments were arranged factorially with glycerol levels of 5, 7, 9 or 11%; semen cooled from 5 to 1 3 0 C in 3.5 (fast), 20 (moderate) or 40 min (slow); and thawed in water at 5 C for 3 min, 35 C for 12 sec, 55 C for 8 sec, 75 C for 7 sec or 90 C for 5 seconds. Fast freezing resulted in greater postthaw motil i ty than moderate (P < .05) or slow rates (P < .01), regardless of thawing method. Glycerol levels of 7 to 11% provided opt imal survival when averaged over rates of freezing and thawing (P < .05). Post-thaw moti l i ty improved as the temperature of the thawing bath was increased from 5 to 55 C (P < .01). Further increases in thawing bath temperature to 90 C did not enhance survival. The post-thaw motil i ty of spermatozoa frozen rapidly in straws and thawed at 55 to 90 C exceeded that for ampules from split-ejaculates frozen in 1.0-ml ampules (P < .01). Semen from one Angus, two Hereford and three Charolais bulls was frozen in a second study at the fast, moderate and slow rates in straws maintained in the horizontal or vertical position. The final extended semen contained 5, 7, 9 or 11% glycerol; and all semen was thawed in 75 C water for 7 seconds. Fast freezing resulted in post-thaw moti l i ty equal to
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ورودعنوان ژورنال:
- Journal of animal science
دوره 41 1 شماره
صفحات -
تاریخ انتشار 1975